Occupations: 13 HCWs and 13 doffing assistants A PLS suspension (25 mL) was aerosolised using a 3‐jet Collison nebuliser (Mesa Laboratories, Inc, Butler, NJ) for 4 min of continuous aerosol generation while the HCW turned 90° every 60 sĤ8 participants were included in the study Fluorescent tracer mixture was applied to PPE using 1000 mL in a pesticide hand sprayer (RL Flo‐Master, Lowell, MI 2000 mL capacity) and 5 sweeping passes of sprayer from head to feet on the front and back of the HCW. PLSs are commonly utilised in aerosol research and were used to simulate pathogensĮxposure simulation: 1. Fluorescent 2‐μm polystyrene latex bead (PLSs) (G0200, Thermo Fisher Scientific, Waltham, MA) diluted in water. The optimised fluorescent slurry consisted of fluorescent powder (Glitter Bug, Brevis Corporation, Salt Lake City, UT 75 mg/mL) in a viscous suspension of grape‐seed oil and water (1:6 oil‐to‐water ratio) 2. For simulation study, what was used for the exposure (virus, fluorescent fluid etc): 1.

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